Intragenic transposition of Ac generates a new allele of the maize P gene

Peterson, T. (October 1990) Intragenic transposition of Ac generates a new allele of the maize P gene. Genetics, 126 (2). pp. 469-76. ISSN 0016-6731 (Print)0016-6731 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1700963

Abstract

The maize P gene is required for the production of red phlobaphene pigments in the pericarp and cob. The P-vv allele, which specifies variegated pericarp and cob, contains an insertion of the transposable element Ac in the P gene. A new P-ovov allele (orange variegated pericarp and cob) was obtained as a single event mutation from P-vv. In contrast to the progenitor P-vv allele, P-ovov provides substantial pericarp and cob pigmentation and produces significant amounts of normal-sized P transcripts. The Ac element is not detectably altered in the P-ovov allele, but it has transposed to a new position within P that is 161 bp distant and inserted in the opposite orientation. This example provides molecular confirmation for the hypothesis that changes in expression of genes bearing insertions of transposable elements can occur via movement of the element to new sites within the gene.

Item Type: Paper
Uncontrolled Keywords: Alleles Base Sequence Blotting, Southern Crosses, Genetic *DNA Transposable Elements *Genes, Plant Molecular Sequence Data Phenotype Pigments, Biological/*genetics RNA/biosynthesis Restriction Mapping Zea mays/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: October 1990
Date Deposited: 29 Mar 2016 16:48
Last Modified: 29 Mar 2016 16:48
PMCID: PMC1204201
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32303

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