The HIV-1 long terminal repeat contains an unusual element that induces the synthesis of short RNAs from various mRNA and snRNA promoters

Ratnasabapathy, R., Sheldon, M., Johal, L., Hernandez, N. (December 1990) The HIV-1 long terminal repeat contains an unusual element that induces the synthesis of short RNAs from various mRNA and snRNA promoters. Genes Dev, 4 (12A). pp. 2061-74. ISSN 0890-9369 (Print)0890-9369 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2269426
DOI: 10.1101/gad.4.12a.2061

Abstract

We describe an unusual element that activates the synthesis of short transcripts from a wide variety of mRNA and small nuclear RNA (snRNA) promoters, including the U6 RNA polymerase III promoter. This inducer of short transcripts (IST) is located between positions -5 and +82 relative to the cap site in the HIV-1 LTR. In the presence of IST, the total transcriptional activity of the different promoters is greatly increased, but the resulting additional RNA molecules are short, ending around position +60. IST is not the RNA target (TAR) for Tat trans-activation; however, because it relies entirely on cellular factors for activity, IST may serve to provide abundant RNA targets for Tat trans-activation without a requirement for full-length viral mRNA expression.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Genes, tat *HIV Long Terminal Repeat HIV-1/*genetics Molecular Sequence Data Nucleic Acid Conformation *Promoter Regions, Genetic RNA Polymerase II/genetics/metabolism RNA Polymerase III/genetics/metabolism RNA, Messenger/metabolism RNA, Small Nuclear/*genetics RNA, Viral/biosynthesis Restriction Mapping *Transcription, Genetic Transcriptional Activation
Subjects: diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date: December 1990
Date Deposited: 28 Mar 2016 19:33
Last Modified: 03 Nov 2017 20:55
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32293

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