The differential influence of protein kinase inhibitors on retinal arbor morphology and eye-specific stripes in the frog retinotectal system

Cline, H. T., Constantine-Paton, M. (June 1990) The differential influence of protein kinase inhibitors on retinal arbor morphology and eye-specific stripes in the frog retinotectal system. Neuron, 4 (6). pp. 899-908. ISSN 0896-6273 (Print)0896-6273 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2361013
DOI: 10.1016/0896-6273(90)90143-4

Abstract

We investigated retinal axon morphology and eye-specific afferent termination zones in the optic tectum of three-eyed tadpoles that were chronically treated with protein kinase inhibitors. The kinase inhibitors sphingosine, H-7, and phorbol ester, which down-regulates protein kinase C with chronic exposure, were applied to the tecta in a slow release plastic, Elvax. In vivo protein phosphorylation assays in drug-treated tadpoles indicated that the treatments decreased 32P incorporation into some protein bands by as much as 60%. Although the drugs did not cause a desegregation of the eye-specific stripes, treated retinal axon arbors covered about half the area covered by untreated arbors or arbors treated with inactive analogs of the drugs. We conclude that eye-specific segregation can be maintained under conditions that markedly alter retinal ganglion cell axon arbor size and that significantly perturb protein phosphorylation. Furthermore, we conclude that the protein kinase(s) that we blocked with these treatments is involved in the growth of axon arbors.

Item Type: Paper
Uncontrolled Keywords: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Animals Axons/drug effects/*physiology Horseradish Peroxidase Isoquinolines/*pharmacology Nerve Tissue Proteins/biosynthesis Phorbol 12,13-Dibutyrate/*pharmacology Phorbol Esters/pharmacology Phosphorylation Piperazines/*pharmacology Polyvinyls/pharmacology *Protein Kinase Inhibitors Rana pipiens Retina/*physiology Retinal Ganglion Cells/drug effects/*physiology Sphingosine/analogs & derivatives/*pharmacology Superior Colliculi/drug effects/enzymology/*physiology Visual Pathways/drug effects/*physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > tissues types and functions > retina
organs, tissues, organelles, cell types and functions > tissues types and functions > visual cortex
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Matt Covey
Date: June 1990
Date Deposited: 05 Apr 2016 14:45
Last Modified: 05 Apr 2016 14:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32274

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