Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetases

Lee, C. C., Craigen, W. J., Muzny, D. M., Harlow, E., Caskey, C. T. (May 1990) Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetases. Proc Natl Acad Sci U S A, 87 (9). pp. 3508-12. ISSN 0027-8424 (Print)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2185472
DOI: 10.1073/pnas.87.9.3508

Abstract

The termination of protein synthesis is encoded by in-frame nonsense (stop) codons. Most organisms use three nonsense codons: UGA, UAG, and UAA. In contrast to sense codons, which are decoded by specific tRNAs, nonsense codons are decoded by proteins called release factors (RFs). Here we report the cloning of a mammalian RF cDNA by the use of monoclonal antibodies specific for rabbit RF. Functional studies showed that, when expressed in Escherichia coli, the protein encoded by this cDNA has in vitro biochemical characteristics similar to those of previously characterized mammalian RFs. DNA sequencing of this eukaryotic RF cDNA revealed a remarkable sequence similarity to bacterial and mitochondrial tryptophanyl-tRNA synthetases, with the greatest similarity confined to the synthetase active site, and no obvious similarity to bacterial RFs.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Amino Acyl-tRNA Synthetases/*genetics Animals Base Sequence Binding Sites Cell Line Cloning, Molecular DNA/genetics Escherichia coli/genetics Gene Library Liver/metabolism Molecular Sequence Data Peptide Termination Factors/*genetics Rabbits Sequence Homology, Nucleic Acid Transfection Tryptophan-tRNA Ligase/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > synthetases
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > tRNA
organism description > virus > viral transfection
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: May 1990
Date Deposited: 06 Apr 2016 15:20
Last Modified: 09 Nov 2017 20:43
PMCID: PMC53930
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32265

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