Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase

D'Urso, G., Marraccino, R. L., Marshak, D. R., Roberts, J. M. (November 1990) Cell cycle control of DNA replication by a homologue from human cells of the p34cdc2 protein kinase. Science, 250 (4982). pp. 786-91. ISSN 0036-8075 (Print)0036-8075 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2173140
DOI: 10.1126/science.2173140

Abstract

The regulation of DNA replication during the eukaryotic cell cycle was studied in a system where cell free replication of simian virus 40 (SV40) DNA was used as a model for chromosome replication. A factor, RF-S, was partially purified from human S phase cells based on its ability to activate DNA replication in extracts from G1 cells. RF-S contained a human homologue of the Schizosaccharomyces pombe p34cdc2 kinase, and this kinase was necessary for RF-S activity. The limiting step in activation of the p34 kinase at the G1 to S transition may be its association with a cyclin since addition of cyclin A to a G1 extract was sufficient to start DNA replication. These observations suggest that the role of p34cdc2 in controlling the start of DNA synthesis has been conserved in evolution.

Item Type: Paper
Uncontrolled Keywords: Burkitt Lymphoma CDC2 Protein Kinase/genetics/*physiology *Cell Cycle Cyclins/pharmacology *DNA Replication Humans Interphase Phosphorylation Schizosaccharomyces/enzymology Simian virus 40/*genetics/physiology Tumor Cells, Cultured *Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 9 November 1990
Date Deposited: 05 Apr 2016 19:57
Last Modified: 05 Apr 2016 19:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32258

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