The angiotensinogen gene of Swiss mice is closely linked to a retrovirus-like element

Clouston, W. M. (November 1990) The angiotensinogen gene of Swiss mice is closely linked to a retrovirus-like element. DNA and Cell Biology, 9 (9). pp. 623-630. ISSN 1044-5498

URL: http://www.ncbi.nlm.nih.gov/pubmed/2175614
DOI: 10.1089/dna.1990.9.623

Abstract

Angiotensinogen is cleaved by renin and angiotensin-converting enzyme to liberate the potent vasoconstrictor peptide angiotensin II. We have recently identified a cis-acting genetic lesion associated with high levels of angiotensinogen mRNA in the testis and salivary gland of Swiss mice. To determine the molecular basis of this mutation, the Swiss angiotensinogen gene was cloned, and its structure was compared to that from a low-expressing strain (BALB/c). I show that a retrovirus-like element belonging to the intracisternal A-particle gene family has been inserted 9 kb upstream from the cap site of the Swiss angiotensinogen gene. This intracisternal A-particle, named IAP-Agt, segregated concordantly with angiotensinogen expression phenotypes in CXB recombinant inbred mice. However, genomic Southern analysis showed that IAP-Agt was present in some, but not all, inbred laboratory mouse strains displaying high levels of angiotensinogen gene expression. On the basis of the evolutionary evidence, it is unlikely that IAP-Agt is the cause of the angiotensinogen mutation. It is intriguing that Ren-2, the duplicated mouse gene, is expressed to high levels in the male salivary gland and also contains a transposed intracisternal A-particle genome.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > transposable elements
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: November 1990
Date Deposited: 06 Apr 2016 16:14
Last Modified: 06 Apr 2016 16:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32252

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