Analysis of E1A-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity

Stein, R. W., Corrigan, M., Yaciuk, P., Whelan, J., Moran, E. (September 1990) Analysis of E1A-mediated growth regulation functions: binding of the 300-kilodalton cellular product correlates with E1A enhancer repression function and DNA synthesis-inducing activity. J Virol, 64 (9). pp. 4421-7. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2143544

Abstract

Adenovirus E1A transforming function requires two distinct regions of the protein. Transforming activity is closely linked with the presence of a region designated conserved domain 2 and the ability of this region to bind the product of the cellular retinoblastoma tumor suppressor gene. We have investigated the biological properties of the second transforming region of E1A, which is located near the N terminus. Transformation-defective mutants containing deletions in the N terminus (deletion of residues between amino acids 2 and 36) were deficient in the ability to induce DNA synthesis and repress insulin enhancer-stimulated activity. The function of the N-terminal region correlated closely with binding of the 300-kilodalton E1A-associated protein and not with binding of the retinoblastoma protein. These results indicate that transformation by E1A is mediated by two functionally independent regions of the protein which interact with different specific cellular proteins and suggest that the 300-kilodalton E1A-associated protein plays a major role in E1A-mediated cell growth control mechanisms.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Adenoviruses, Human/*genetics Animals Antibodies, Monoclonal Cell Line Cell Transformation, Viral Chromosome Deletion *DNA Replication DNA, Viral/genetics DNA-Binding Proteins/*metabolism *Enhancer Elements, Genetic HeLa Cells/cytology Humans Insulin/genetics Molecular Weight Mutation Oncogene Proteins, Viral/genetics/*metabolism RNA, Messenger/genetics Repressor Proteins/*metabolism Thymidine/metabolism Transcription Factors/*metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: September 1990
Date Deposited: 06 Apr 2016 16:03
Last Modified: 06 Apr 2016 16:03
PMCID: PMC247911
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32250

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