The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae

Surana, U., Robitsch, H., Price, C., Schuster, T., Fitch, I., Futcher, A. B., Nasmyth, K. (April 1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell, 65 (1). pp. 145-61. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1849457
DOI: 10.1016/0092-8674(91)90416-V

Abstract

cdc28-1N is a conditional allele that has normal G1 (Start) function but confers a mitotic defect. We have isolated seven genes that in high dosage suppress the growth defect of cdc28-1N cells but not of Start-defective cdc28-4 cells. Three of these (CLB1, CLB2, and CLB4) encode proteins strongly homologous to G2-specific B-type cyclins. Another gene, CLB3, was cloned using PCR, CLB1 and CLB2 encode a pair of closely related proteins; CLB3 and CLB4 encode a second pair. Neither CLB1 nor CLB2 is essential; however, disruption of both is lethal and causes a mitotic defect. Furthermore, the double mutant cdc28-1N clb2::LEU2 is nonviable, whereas cdc28-4 clb2::LEU2 is viable, suggesting that the cdc28-1N protein may be defective in its interaction with B-type cyclins. Our results are consistent with CDC28 function being required in both G1 and mitosis. Its mitotic role, we believe, involves interaction with a family of at least four G2-specific cyclins.

Item Type: Paper
Uncontrolled Keywords: Alleles Amino Acid Sequence Base Sequence Cloning, Molecular Cyclins/genetics/*physiology DNA, Fungal Gene Expression Regulation, Fungal Genes, Fungal Genes, Suppressor Mitosis/*physiology Molecular Sequence Data Mutation Phenotype Plasmids Polymerase Chain Reaction Protamine Kinase/metabolism Protein Kinases/genetics/*physiology Restriction Mapping Saccharomyces cerevisiae/genetics/*physiology Sequence Homology, Nucleic Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Futcher lab
Depositing User: Matt Covey
Date: 5 April 1991
Date Deposited: 16 Dec 2015 21:03
Last Modified: 16 Dec 2015 21:03
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32136

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