Surana, U., Robitsch, H., Price, C., Schuster, T., Fitch, I., Futcher, A. B., Nasmyth, K. (April 1991) The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell, 65 (1). pp. 145-61. ISSN 0092-8674 (Print)0092-8674 (Linking)
Abstract
cdc28-1N is a conditional allele that has normal G1 (Start) function but confers a mitotic defect. We have isolated seven genes that in high dosage suppress the growth defect of cdc28-1N cells but not of Start-defective cdc28-4 cells. Three of these (CLB1, CLB2, and CLB4) encode proteins strongly homologous to G2-specific B-type cyclins. Another gene, CLB3, was cloned using PCR, CLB1 and CLB2 encode a pair of closely related proteins; CLB3 and CLB4 encode a second pair. Neither CLB1 nor CLB2 is essential; however, disruption of both is lethal and causes a mitotic defect. Furthermore, the double mutant cdc28-1N clb2::LEU2 is nonviable, whereas cdc28-4 clb2::LEU2 is viable, suggesting that the cdc28-1N protein may be defective in its interaction with B-type cyclins. Our results are consistent with CDC28 function being required in both G1 and mitosis. Its mitotic role, we believe, involves interaction with a family of at least four G2-specific cyclins.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Alleles Amino Acid Sequence Base Sequence Cloning, Molecular Cyclins/genetics/*physiology DNA, Fungal Gene Expression Regulation, Fungal Genes, Fungal Genes, Suppressor Mitosis/*physiology Molecular Sequence Data Mutation Phenotype Plasmids Polymerase Chain Reaction Protamine Kinase/metabolism Protein Kinases/genetics/*physiology Restriction Mapping Saccharomyces cerevisiae/genetics/*physiology Sequence Homology, Nucleic Acid |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs > Futcher lab |
| Depositing User: | Matt Covey |
| Date: | 5 April 1991 |
| Date Deposited: | 16 Dec 2015 21:03 |
| Last Modified: | 16 Dec 2015 21:03 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32136 |
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