Quantitative analysis of protein synthesis in mouse embryos. I. Extensive reprogramming at the one- and two-cell stages

Latham, K. E., Garrels, J. I., Chang, C., Solter, D. (August 1991) Quantitative analysis of protein synthesis in mouse embryos. I. Extensive reprogramming at the one- and two-cell stages. Development, 112 (4). pp. 921-932. ISSN 0950-1991

URL: http://www.ncbi.nlm.nih.gov/pubmed/1935701

Abstract

A quantitative, high-resolution, two-dimensional gel protein database has been constructed for the mouse embryo. This database has been used to obtain a detailed accounting of the amount and relative timing of changes in protein synthesis that occur during the 1-, 2-, and 4-cell stages along with a description of the most prevalent developmentally regulated patterns of synthesis. We find that during these early stages of development the pattern of proteins synthesized changes to a much greater extent than previously appreciated. During the 1- and 2-cell stages, the majority (60 % and 85 %, respectively) of the analyzed proteins exhibit twofold or greater changes in their rates of synthesis. The periods of greatest change are the late 1-cell and mid 2-cell stages, during which an extensive remodelling of protein synthetic pattern occurs that is largely complete by 15 h following the first cleavage. Once this reprogramming is complete, very little change is observed during the late 2-cell and 4-cell stages. Cluster analysis of individual protein synthesis patterns reveals a limited number of coordinately regulated protein sets that are responsible for most of the changes observed during the 1- and 2-cell stages. During the 2-cell stage, one third of the proteins increase by an average of fivefold, another third decrease by an average of sevenfold, and 10 % undergo transient changes in rates of synthesis. These patterns reflect the switch from zygotic to maternal mRNA utilization following transcriptional activation. Significantly, a set of proteins exhibiting relatively constant rates of synthesis is not observed during the 2-cell stage, indicating that the rate of synthesis of almost every detectable protein changes significantly during this period. Overall, our data indicate that the transition from maternal to embryonic control of development results in an abrupt and extensive reprogramming of the early embryo with respect to the pattern of proteins synthesized.

Item Type: Paper
Uncontrolled Keywords: MOUSE EMBRYO PROTEIN SYNTHESIS PROTEIN DATABASE 2-DIMENSIONAL GEL ELECTROPHORESIS POST-TRANSCRIPTIONAL CONTROL PRE-IMPLANTATION DEVELOPMENT ACTIN MESSENGER-RNA POLYADENYLATED RNA GENE-EXPRESSION PATTERNS ADENYLATION CLEAVAGE OOCYTES HISTONE
Subjects: organism description > animal > developmental stage
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: August 1991
Date Deposited: 21 Dec 2015 20:30
Last Modified: 21 Dec 2015 20:30
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32126

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