Kidney function in ANF-transgenic mice: effect of blood volume expansion

Field, L. J., Veress, A. T., Steinhelper, M. E., Cochrane, K., Sonnenberg, H. (January 1991) Kidney function in ANF-transgenic mice: effect of blood volume expansion. Am J Physiol, 260 (1 Pt 2). R1-5. ISSN 0002-9513 (Print)0002-9513 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1825155

Abstract

Transgenic mice, created from inbred C3HeB/FeJ embryos, were used to overexpress selectively in the liver a fusion gene comprising mouse transthyretin (TTR) regulatory and atrial natriuretic factor (ANF) structural sequences. Animals were anesthetized, and kidney function was studied before and after blood volume expansion. Baseline urine volumes and electrolyte excretions were not significantly different from those of non-transgenic littermates, despite a markedly lower arterial blood pressure in the experimental group. A slightly lower glomerular filtration rate (GFR) in transgenics was not different statistically. Plasma ANF levels measured by radioimmunoassay were approximately 10-fold higher in the transgenic animals, compared with their nontransgenic siblings. After acute blood volume expansion, the diuretic, natriuretic, kaliuretic, and chloruretic responses were markedly enhanced in the transgenic group. Arterial pressure was increased as a result of hypervolemia, although it remained relatively depressed relative to the controls. GFR again was not different. We conclude that transgenic mice overexpressing ANF can maintain normal excretion of salt and water, possibly via ANF-induced reduction of renal perfusion pressure. After acute blood volume expansion, an increase in pressure may allow full renal expression of the chronically elevated ANF levels.

Item Type: Paper
Uncontrolled Keywords: Animals Atrial Natriuretic Factor/blood/*genetics/physiology Blood Pressure/drug effects/physiology Blood Volume/drug effects/physiology Chlorides/urine Gene Expression Glomerular Filtration Rate/drug effects/physiology Kidney/metabolism/*physiology Mice Mice, Inbred Strains Mice, Transgenic Potassium/urine Prealbumin/genetics/metabolism/physiology Radioimmunoassay Sodium/urine
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > red blood cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > red blood cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > red blood cells

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: January 1991
Date Deposited: 22 Dec 2015 21:38
Last Modified: 22 Dec 2015 21:38
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32100

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