Isolation and sequence analysis of TGA1 cDNAs encoding a tomato G protein alpha subunit

Ma, H., Yanofsky, M. F., Huang, H. (November 1991) Isolation and sequence analysis of TGA1 cDNAs encoding a tomato G protein alpha subunit. Gene, 107 (2). pp. 189-95. ISSN 0378-1119 (Print)0378-1119 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1748292
DOI: 10.1016/0378-1119(91)90318-6

Abstract

We have isolated cDNAs for a gene coding for a G protein alpha subunit from tomato (Lycopersicon esculentum, cv. VF36). This gene, named TGA1, was isolated using a cDNA of the Arabidopsis thaliana G protein alpha subunit-encoding gene, GPA1, as a DNA probe. The sequences of four cDNA clones indicate that the deduced amino acid (aa) sequence of the gene product (TG alpha 1) has 384 aa (44906 Da). The predicted TG alpha 1 protein exhibits similarity to all known G protein alpha subunits. The aa are 84.6% identical and 93% similar (identical and conservative changes) to A. thaliana GP alpha 1, and 34% identical and 59% similar to mammalian transducins. Furthermore, it has all of the consensus regions for a GTP-binding protein. Finally, hybridizations of tomato genomic DNA indicate that TGA1 is a single-copy gene.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular Consensus Sequence GTP-Binding Proteins/*genetics Molecular Sequence Data Plant Proteins/*genetics Plants/*genetics Restriction Mapping Sequence Alignment
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 November 1991
Date Deposited: 22 Dec 2015 21:29
Last Modified: 22 Dec 2015 21:29
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32099

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