Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays

Colasanti, J., Tyers, M., Sundaresan, V. (April 1991) Isolation and characterization of cDNA clones encoding a functional p34cdc2 homologue from Zea mays. Proc Natl Acad Sci U S A, 88 (8). pp. 3377-81. ISSN 0027-8424 (Print)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2014258

Abstract

We describe the isolation of cDNA clones encoding a p34cdc2 homologue from a higher plant, Zea mays (maize). A full-length cDNA clone, cdc2ZmA, was isolated, sequenced, and shown to complement a cdc28 mutation in Saccharomyces cerevisiae. Comparison of the deduced amino acid sequence of the maize p34cdc2 protein with other homologues showed that it was 64% identical to human p34cdc2 and 63% identical to Schizosaccharomyces pombe and S. cerevisiae p34cdc2 proteins. Studies of expression of the maize cdc2 gene(s) by Northern blot analysis indicated a correlation between the abundance of cdc2 mRNA and the proliferative state of the tissue. Southern blot analysis, as well as isolation of another cDNA clone, cdc2ZmB, which is 96% identical to cdc2ZmA, indicates that maize has multiple cdc2 genes.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern CDC2 Protein Kinase/*genetics *Cell Cycle Cloning, Molecular DNA/genetics Gene Expression Genes Genetic Complementation Test Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Schizosaccharomyces/genetics Zea mays/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organism description > plant
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 April 1991
Date Deposited: 22 Dec 2015 20:42
Last Modified: 09 Nov 2017 20:19
PMCID: PMC51450
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32098

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