The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: evidence for a homeo domain recognition subdomain

Stern, S., Herr, W. (December 1991) The herpes simplex virus trans-activator VP16 recognizes the Oct-1 homeo domain: evidence for a homeo domain recognition subdomain. Genes Dev, 5 (12B). pp. 2555-66. ISSN 0890-9369 (Print)0890-9369 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/1684335
DOI: 10.1101/gad.5.12b.2555

Abstract

The homeo domain of the Oct-1 transcription factor directs formation of a multiprotein-DNA complex containing Oct-1, the herpes simplex virus (HSV) trans-activator VP16, and a second host cell factor (HCF). This VP16-induced complex alters the regulatory activity of Oct-1, in part, by associating it with the potent VP16 acidic transcriptional activation domain. Here, we show that in the absence of HCF, VP16 can recognize specifically the Oct-1 homeo domain. A region of VP16 near the acidic activation domain appears to be involved exclusively in homeo domain recognition because a 4-amino-acid insertion within this region only affects the ability of VP16 to interact with Oct-1, leaving its DNA- and HCF-binding activities unchanged. A 33-amino-acid peptide containing this region complexes with the Oct-1 POU domain bound to DNA, suggesting that this VP16 region contains an autonomous homeo domain recognition subdomain.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Carrier Proteins/genetics DNA, Viral/chemistry DNA-Binding Proteins/*genetics *Genes, Homeobox Herpes Simplex Virus Protein Vmw65 Host Cell Factor C1 Integration Host Factors Molecular Sequence Data Mutation Octamer Transcription Factor-1 Simplexvirus/*genetics Trans-Activators/*genetics Transcription Factors/*genetics Transcription, Genetic Viral Proteins/*genetics
Subjects: organism description > virus > herpes simplex virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > homeodomain protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: December 1991
Date Deposited: 07 Jan 2016 17:11
Last Modified: 03 Nov 2017 20:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32084

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