Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice

Skowronski, J. (February 1991) Expression of a human immunodeficiency virus type 1 long terminal repeat/simian virus 40 early region fusion gene in transgenic mice. J Virol, 65 (2). pp. 754-62. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1846196

Abstract

Three lines of transgenic mice carrying the human immunodeficiency virus type 1 (HIV-1) long terminal repeat fused to the simian virus 40 early region (HIV-1 Tag) were constructed. Expression of the transgenes was reproducibly observed in the lymphoid tissue and skin of all three transgenic lines studied. Interestingly, cell types other than T cells, i.e., B cells and thymic stromal cells, contributed most of the expression detectable in the lymphoid organs. Each transgenic line also displayed a different but consistent pattern of transgene expression in nonlymphoid organs. These individual patterns probably reflect the effects of particular chromosomal integration sites on transcriptional activity of the HIV-1 promoter.

Item Type: Paper
Uncontrolled Keywords: Animals B-Lymphocytes/metabolism Base Sequence Cloning, Molecular *Genes, Viral HIV Long Terminal Repeat/*genetics HIV-1/*genetics Mice Mice, Inbred Strains Mice, Transgenic Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Promoter Regions, Genetic RNA, Messenger/genetics RNA, Viral/genetics/isolation & purification Simian virus 40/*genetics T-Lymphocytes/metabolism Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
CSHL Authors:
Communities: CSHL labs > Skowronski lab
Depositing User: Matt Covey
Date: February 1991
Date Deposited: 11 Jan 2016 16:40
Last Modified: 11 Jan 2016 16:40
PMCID: PMC239815
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32073

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving