Domains of the adenovirus E1A protein required for oncogenic activity are also required for dissociation of E2F transcription factor complexes

Raychaudhuri, P., Bagchi, S., Devoto, S. H., Kraus, V. B., Moran, E., Nevins, J. R. (July 1991) Domains of the adenovirus E1A protein required for oncogenic activity are also required for dissociation of E2F transcription factor complexes. Genes & Development, 5 (7). pp. 1200-1211. ISSN 0890-9369

URL: http://www.ncbi.nlm.nih.gov/pubmed/1829698
DOI: 10.1101/gad.5.7.1200

Abstract

Recent experiments have shown that the cellular E2F transcription factor is found in complexes with cellular proteins and that one such complex contains the cyclin-A protein. Isolation of a cellular activity, which we term E2F-BF, can reconstitute the E2F-cyclin-A complex and has permitted a more detailed analysis of the mechanism of E1A dissociation. Through the analysis of a series of E1A mutants, we find that sequences in conserved region 1 (CR1) and conserved region 2 (CR2) are important for dissociation of the E2F complex, whereas amino-terminal sequences are not required. In contrast to the requirements for dissociation, only the CRI sequences are required to block formation of the complex if E1A is added when the components are combined. We have also identified an activity, termed E2F-I, that inhibits E2F binding to DNA, again apparently through the formation of a complex with E2F. This inhibitory activity is also blocked by E1A, dependent on the same elements of the E1A protein that disrupt the interaction with E2F-BF. Because the E1A sequences that are important for releasing E2F from these interactions are also sequences necessary for oncogenesis, we suggest that this activity may be a critical component of the transforming activity of E1A.

Item Type: Paper
Uncontrolled Keywords: e2f-bf cyclin-a adenovirus e1a protein oncogenic activity e2f transcription factor DNA-binding activity gene-products trans-activation messenger-rna e4 gene individual products functional domains cellular proteins terminal region transformation
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: July 1991
Date Deposited: 13 Jan 2016 19:12
Last Modified: 13 Jan 2016 19:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32068

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