Distinct protein targets for signals acting at the c-fos serum response element

Graham, R., Gilman, M. (1991) Distinct protein targets for signals acting at the c-fos serum response element. Science, 251 (4990). pp. 189-92. ISSN 0036-8075 (Print)0036-8075 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1898992
DOI: 10.1126/science.1898992

Abstract

The c-fos serum response element (SRE) is a primary nuclear target for intracellular signal transduction pathways triggered by growth factors. It is the target for both protein kinase C (PKC)-dependent and -independent signals. Function of the SRE requires binding of a cellular protein, termed serum response factor (SRF). A second protein, p62TCF, recognizes the SRE-SRF complex to form a ternary complex. A mutated SRE that bound SRF but failed to form the ternary complex selectively lost response to PKC activators, but retained response to PKC-independent signals. Thus, two different signaling pathways act through discrete nuclear targets at the SRE. At least one of these pathways functions by recruitment of a pathway-specific accessory factor (p62TCF). These results offer a molecular mechanism to account for the biological specificity of signals that appear to act through common DNA sequence elements.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites Blood Cell Line DNA/metabolism *Enhancer Elements, Genetic Mice Mice, Inbred BALB C Molecular Sequence Data Mutagenesis Nuclear Proteins/*metabolism Promoter Regions, Genetic/genetics Protein Kinase C/metabolism Proto-Oncogene Proteins/*genetics Proto-Oncogene Proteins c-fos Second Messenger Systems Serum Response Factor *Signal Transduction Tetradecanoylphorbol Acetate/pharmacology Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 13 Jan 2016 17:35
Last Modified: 13 Jan 2016 17:35
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32067

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