Graham, R., Gilman, M. (January 1991) Distinct protein targets for signals acting at the c-fos serum response element. Science, 251 (4990). pp. 189-92. ISSN 0036-8075 (Print)0036-8075 (Linking)
Abstract
The c-fos serum response element (SRE) is a primary nuclear target for intracellular signal transduction pathways triggered by growth factors. It is the target for both protein kinase C (PKC)-dependent and -independent signals. Function of the SRE requires binding of a cellular protein, termed serum response factor (SRF). A second protein, p62TCF, recognizes the SRE-SRF complex to form a ternary complex. A mutated SRE that bound SRF but failed to form the ternary complex selectively lost response to PKC activators, but retained response to PKC-independent signals. Thus, two different signaling pathways act through discrete nuclear targets at the SRE. At least one of these pathways functions by recruitment of a pathway-specific accessory factor (p62TCF). These results offer a molecular mechanism to account for the biological specificity of signals that appear to act through common DNA sequence elements.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Base Sequence Binding Sites Blood Cell Line DNA/metabolism *Enhancer Elements, Genetic Mice Mice, Inbred BALB C Molecular Sequence Data Mutagenesis Nuclear Proteins/*metabolism Promoter Regions, Genetic/genetics Protein Kinase C/metabolism Proto-Oncogene Proteins/*genetics Proto-Oncogene Proteins c-fos Second Messenger Systems Serum Response Factor *Signal Transduction Tetradecanoylphorbol Acetate/pharmacology Transfection |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 11 January 1991 |
| Date Deposited: | 13 Jan 2016 17:35 |
| Last Modified: | 13 Jan 2016 17:35 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32067 |
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