Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors

Grotewold, E., Athma, P., Peterson, T. (June 1991) Alternatively spliced products of the maize P gene encode proteins with homology to the DNA-binding domain of myb-like transcription factors. Proc Natl Acad Sci U S A, 88 (11). pp. 4587-91. ISSN 0027-8424 (Print)0027-8424 (Linking)

[img]
Preview
PDF (Paper)
Grotewald PNAS 1991.pdf - Published Version

Download (1381Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/2052542

Abstract

The Zea mays P gene has been postulated to regulate the biosynthetic pathway of a flavonoid-derived pigment in certain floral tissues [Styles, E. D. & Ceska, O. (1977) Can. J. Genet. Cytol. 19, 289-302]. We have characterized two P transcripts that are alternatively spliced at their 3' ends. One message of 1802 nucleotides encodes a 43.7-kDa protein with an N-terminal region showing approximately 40% homology to the DNA-binding domain of several members of the myb family of protooncogene proteins. A second message of 945 nucleotides encodes a 17.3-kDa protein that contains most of the myb-homologous domain but differs from the first protein at the C terminus. The deduced P-encoded proteins show an even higher homology (70%) in the myb-homologous domain to the maize regulatory gene C1. Additionally, the P and C1 genes are structurally similar in the sizes and positions of the first and second exons and first intron. We show that P is required for accumulation in the pericarp of transcripts of two genes (A1 and C2) encoding enzymes for flavonoid biosynthesis--genes also regulated by C1 in the aleurone.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular DNA-Binding Proteins/*genetics *Genes, Plant Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plant Proteins/*genetics Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/*genetics Proto-Oncogene Proteins c-myb *RNA Splicing Restriction Mapping Sequence Homology, Nucleic Acid Transcription Factors/*genetics Transcription, Genetic Zea mays/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Grotewold lab
Depositing User: Matt Covey
Date: 1 June 1991
Date Deposited: 14 Jan 2016 16:50
Last Modified: 09 Nov 2017 20:19
PMCID: PMC51710
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32039

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving