CFLIP expression is altered in severe corticosteroid-resistant asthma

Lazarev, D., Miller, R. L., Dimango, E., Fu, X. D., Li, H. R., Logan, C. J., Manley, J. L. (May 2014) CFLIP expression is altered in severe corticosteroid-resistant asthma. Genomics Data, 2. pp. 99-104. ISSN 22135960

URL: http://www.ncbi.nlm.nih.gov/pubmed/26484081
DOI: 10.1016/j.gdata.2014.05.003

Abstract

Dysregulation of alternative splicing of mRNA precursors is known to contribute to numerous human diseases. In this study we carried out the first systematic search for asthma-associated changes in alternative splicing events, using a model of Aspergillus fumigatus (A. fumigatus)-sensitized mice and an exon junction microarray to detect potential changes in alternative splicing. One of the sensitization-associated changes identified in the search was a shift in alternative splicing of the mRNA encoding cFLIP, a modulator of the caspase-mediated extrinsic apoptosis pathway. Expanding these studies to human asthma patients, we discovered a significant decrease in the expression of both cFLIP isoforms in severe corticosteroid-resistant asthmatics. Although it is unclear whether these changes were due solely to differences in alternative splicing, these findings provide evidence that dysregulation of the extrinsic apoptosis pathway is part of the underlying immunopathogenesis of severe refractory asthma.

Item Type: Paper
Uncontrolled Keywords: Alternative splicing Asthma Microarrays
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Alternative Splicing
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > Protease > caspases
CSHL Authors:
Communities: CSHL labs > Joshua-Tor lab
Depositing User: Matt Covey
Date: 23 May 2014
Date Deposited: 27 Oct 2015 20:04
Last Modified: 27 Oct 2015 20:04
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31952

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