Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization

Dequeant, M. L., Fagegaltier, D., Hu, Y., Spirohn, K., Simcox, A., Hannon, G. J., Perrimon, N. (October 2015) Discovery of progenitor cell signatures by time-series synexpression analysis during Drosophila embryonic cell immortalization. Proc Natl Acad Sci U S A, 112 (42). pp. 12974-12979. ISSN 1091-6490 (Electronic)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/26438832
DOI: 10.1073/pnas.1517729112

Abstract

The use of time series profiling to identify groups of functionally related genes (synexpression groups) is a powerful approach for the discovery of gene function. Here we apply this strategy during RasV12 immortalization of Drosophila embryonic cells, a phenomenon not well characterized. Using high-resolution transcriptional time-series datasets, we generated a gene network based on temporal expression profile similarities. This analysis revealed that common immortalized cells are related to adult muscle precursors (AMPs), a stem cell-like population contributing to adult muscles and sharing properties with vertebrate satellite cells. Remarkably, the immortalized cells retained the capacity for myogenic differentiation when treated with the steroid hormone ecdysone. Further, we validated in vivo the transcription factor CG9650, the ortholog of mammalian Bcl11a/b, as a regulator of AMP proliferation predicted by our analysis. Our study demonstrates the power of time series synexpression analysis to characterize Drosophila embryonic progenitor lines and identify stem/progenitor cell regulators.

Item Type: Paper
Uncontrolled Keywords: Drosophila RNA-Seq immortalization time series
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell

Investigative techniques and equipment > assays > RNA-seq
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 5 October 2015
Date Deposited: 20 Oct 2015 20:26
Last Modified: 08 Nov 2017 20:08
PMCID: PMC4620889
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31932

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