Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA

Chandran, D., Scanlon, M. J., Ohtsu, K., Timmermans, M. C., Schnable, P. S., Wildermuth, M. C. (October 2015) Laser Microdissection-Mediated Isolation and In Vitro Transcriptional Amplification of Plant RNA. Curr Protoc Mol Biol, 112. 25A 3.1-25A 3.23. ISSN 1934-3647 (Electronic)1934-3647 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/26423587
DOI: 10.1002/0471142727.mb25a03s112

Abstract

Laser microdissection of cells allows for isolation of specific cells of interest for downstream analyses including transcriptional profiling. Plant cells present unique challenges for laser microdissection due to their cellulosic cell walls and large vacuoles. Here we present protocols for plant tissue preparation, laser microdissection of select plant cells, and linear amplification of RNA from dissected cells. Linear amplification of RNA from dissected cells allows sufficient RNA for subsequent quantitative analysis by RT-PCR, microarray, or RNA sequencing. (c) 2015 by John Wiley & Sons, Inc.

Item Type: Paper
Uncontrolled Keywords: RNA amplification laser microdissection plants transcriptomics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: Matt Covey
Date: 1 October 2015
Date Deposited: 20 Oct 2015 20:21
Last Modified: 20 Oct 2015 20:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31931

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