Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle

Koff, A., Giordano, A., Desai, D., Yamashita, K., Harper, J. W., Elledge, S., Nishimoto, T., Morgan, D. O., Franza, B. R., Roberts, J. M. (September 1992) Formation and activation of a cyclin E-cdk2 complex during the G1 phase of the human cell cycle. Science, 257 (5077). pp. 1689-94. ISSN 0036-8075 (Print)0036-8075 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1388288
DOI: 10.1126/science.1388288

Abstract

Human cyclin E, originally identified on the basis of its ability to function as a G1 cyclin in budding yeast, associated with a cell cycle-regulated protein kinase in human cells. The cyclin E-associated kinase activity peaked during G1, before the appearance of cyclin A, and was diminished during exit from the cell cycle after differentiation or serum withdrawal. The major cyclin E-associated kinase in human cells was Cdk2 (cyclin-dependent kinase 2). The abundance of the cyclin E protein and the cyclin E-Cdk2 complex was maximal in G1 cells. These results provide further evidence that in all eukaryotes assembly of a cyclin-Cdk complex is an important step in the biochemical pathway that controls cell proliferation during G1.

Item Type: Paper
Uncontrolled Keywords: Animals B-Lymphocytes/metabolism *CDC2-CDC28 Kinases Cell Line Cyclin-Dependent Kinase 2 *Cyclin-Dependent Kinases Cyclins/*metabolism Flow Cytometry G1 Phase/*physiology Humans Immunoblotting Immunosorbent Techniques Protein Kinases/*metabolism *Protein-Serine-Threonine Kinases Rats
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 18 September 1992
Date Deposited: 18 Sep 2015 19:01
Last Modified: 18 Sep 2015 19:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31865

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