Characterization of Arabidopsis thaliana telomeres isolated in yeast

Richards, E. J., Chao, S., Vongs, A., Yang, J. (August 1992) Characterization of Arabidopsis thaliana telomeres isolated in yeast. Nucleic Acids Res, 20 (15). pp. 4039-46. ISSN 0305-1048 (Print)0305-1048 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/1508688
DOI: 10.1093/nar/20.15.4039

Abstract

In an effort to learn more about the genomic organization of chromosomal termini in plants we employed a functional complementation strategy to isolate Arabidopsis thaliana telomeres in the yeast, Saccharomyces cerevisiae. Eight yeast episomes carrying A. thaliana telomeric sequences were obtained. The plant sequences carried on two episomes, YpAtT1 and YpAtT7, were characterized in detail. The telomeric origins of YpAtT1 and YpAtT7 insert DNAs were confirmed by demonstrating that corresponding genomic sequences are preferentially degraded during exonucleolytic digestion. The isolated telomeric restriction fragments contain G-rich repeat arrays characteristic of A. thaliana telomeres, as well as subterminal telomere-associated sequences (TASs). DNA sequence analysis revealed the presence of variant telomeric repeats at the centromere-proximal border of the terminal block of telomere repeats. The TAS flanking the telomeric G-rich repeat in YpAtT7 corresponds to a repetitive element present at other A. thaliana telomeres, while more proximal sequences are unique to one telomere. The YpAtT1 TAS is unique in the Landsberg strain of A. thaliana from which the clone originated; however, the Landsberg TAS cross-hybridizes weakly to a second telomere in the strain Columbia. Restriction analysis with cytosine methylation-sensitive endonucleases indicated that both TASs are highly methylated in the genome.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Blotting, Southern Cloning, Molecular Genetic Complementation Test Methylation Molecular Sequence Data Plants/*genetics Plasmids/genetics Repetitive Sequences, Nucleic Acid/*genetics Saccharomyces cerevisiae/genetics *Telomere
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 11 August 1992
Date Deposited: 23 Sep 2015 16:49
Last Modified: 08 Nov 2017 16:15
PMCID: PMC334085
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31843

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