ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex

Bell, S. P., Stillman, B. (1992) ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature, 357 (6374). pp. 128-34. ISSN 0028-0836 (Print)0028-0836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1579162
DOI: 10.1038/357128a0

Abstract

A multiprotein complex that specifically recognizes cellular origins of DNA replication has been identified and purified from the yeast Saccharomyces cerevisiae. We observe a strong correlation between origin function and origin recognition by this activity. Interestingly, specific DNA binding by the origin recognition complex is dependent upon the addition of ATP. We propose that the origin recognition complex acts as the initiator protein for S. cerevisiae origins of DNA replication.

Item Type: Paper
Uncontrolled Keywords: Adenosine Triphosphate/*metabolism Base Sequence Cell Nucleus/metabolism Centrifugation, Density Gradient Chromatography, Affinity *DNA Replication DNA, Fungal/genetics/isolation & purification/metabolism DNA-Binding Proteins/genetics/isolation & purification/*metabolism/radiation effects Electrophoresis, Polyacrylamide Gel Macromolecular Substances Molecular Sequence Data Multiprotein Complexes Oligodeoxyribonucleotides Saccharomyces cerevisiae/*genetics/metabolism Ultraviolet Rays
Subjects: bioinformatics > genomics and proteomics > small molecules > ATP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > eukaryote
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: Matt Covey
Date Deposited: 22 Sep 2015 20:49
Last Modified: 20 Jun 2017 20:03
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31835

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