Promoter-Specific Transactivation by the Adenovirus-E1a12s Product Involves Separate E1a Domains

Kraus, V. B., Moran, E., Nevins, J. R. (1992) Promoter-Specific Transactivation by the Adenovirus-E1a12s Product Involves Separate E1a Domains. Molecular and Cellular Biology, 12 (10). pp. 4391-4399. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/1406628
DOI: 10.1128/MCB.12.10.4391

Abstract

Recent studies have shown that the adenovirus E1A12S product can trans-activate transcription by activating the transcription factor E2F. However, E2F cannot be the only target for the E1A12S product, since several cellular promoters have been found to be activated by the E1A12S protein even though they lack E2F sites. Indeed, we now show that activation of the hsp70 promoter by the E1A12S product requires the TATAA sequence. Moreover, activation of the hsp70 promoter requires the N-terminal domain of the EIA protein and does not require the conserved region 2 sequences which are required for the E2F-dependent activation of transcription. We conclude that the targeting of distinct transcription factors, leading to trans-activation of transcription of multiple promoters, involves distinct domains of the EIA proteins that are also required for oncogenic activity.

Item Type: Paper
Uncontrolled Keywords: E2F TRANSCRIPTION FACTOR RETINOBLASTOMA GENE-PRODUCT CELL NUCLEAR ANTIGEN DNA-BINDING ACTIVITY HUMAN CYCLIN-A FUNCTIONAL DOMAINS TERMINAL REGION MESSENGER-RNAS HSP70 PROMOTER TATA ELEMENTS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 25 Sep 2015 18:51
Last Modified: 25 Sep 2015 18:51
PMCID: PMC360363
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31828

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