The retinoblastoma protein physically associates with the human cdc2 kinase

Hu, Q. J., Lees, J. A., Buchkovich, K. J., Harlow, E. (March 1992) The retinoblastoma protein physically associates with the human cdc2 kinase. Mol Cell Biol, 12 (3). pp. 971-80. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1545827
DOI: 10.1128/MCB.12.3.971

Abstract

The protein product (pRB) of the retinoblastoma susceptibility gene functions as a negative regulator of cell proliferation, and its activity appears to be modulated by phosphorylation. Using a new panel of anti-human pRB monoclonal antibodies, we have investigated the biochemical properties of this protein. These antibodies have allowed us to detect a pRB-associated kinase that has been identified as the cell cycle-regulating kinase p34cdc2 or a closely related enzyme. Since this associated kinase phosphorylates pRB at most of the sites used in vivo, these results suggest that this kinase is one of the major regulators of pRB. The associated kinase activity follows the pattern of phosphorylation seen for pRB in vivo. The associated kinase activity is not seen in the G1 phase but appears in the S phase, and the levels continue to increase throughout the remainder of the cell cycle.

Item Type: Paper
Uncontrolled Keywords: Antibodies, Monoclonal Blotting, Western CDC2 Protein Kinase/*metabolism Cell Division Cells, Cultured Electrophoresis, Gel, Two-Dimensional Humans Peptide Mapping Phosphorylation Precipitin Tests Retinoblastoma Protein/immunology/*metabolism Substrate Specificity
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: March 1992
Date Deposited: 23 Sep 2015 20:02
Last Modified: 23 Sep 2015 20:02
PMCID: PMC369529
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31823

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