Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae

Fitch, I., Dahmann, C., Surana, U., Amon, A., Nasmyth, K., Goetsch, L., Byers, B., Futcher, B. (July 1992) Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. Mol Biol Cell, 3 (7). pp. 805-18. ISSN 1059-1524 (Print)1059-1524 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1387566
DOI: 10.1091/mbc.3.7.805

Abstract

The previously described CLB1 and CLB2 genes encode a closely related pair of B-type cyclins. Here we present the sequences of another related pair of B-type cyclin genes, which we term CLB3 and CLB4. Although CLB1 and CLB2 mRNAs rise in abundance at the time of nuclear division, CLB3 and CLB4 are turned on earlier, rising early in S phase and declining near the end of nuclear division. When all possible single and multiple deletion mutants were constructed, some multiple mutations were lethal, whereas all single mutants were viable. All lethal combinations included the clb2 deletion, whereas the clb1 clb3 clb4 triple mutant was viable, suggesting a key role for CLB2. The inviable multiple clb mutants appeared to have a defect in mitosis. Conditional clb mutants arrested as large budded cells with a G2 DNA content but without any mitotic spindle. Electron microscopy showed that the spindle pole bodies had duplicated but not separated, and no spindle had formed. This suggests that the Clb/Cdc28 kinase may have a relatively direct role in spindle formation. The two groups of Clbs may have distinct roles in spindle formation and elongation.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cloning, Molecular Cyclins/classification/*genetics DNA, Fungal/genetics Gene Expression *Genes, Fungal *Mitosis Molecular Sequence Data RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/*genetics Sequence Alignment
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Futcher lab
Depositing User: Matt Covey
Date: July 1992
Date Deposited: 29 Sep 2015 14:33
Last Modified: 29 Sep 2015 14:33
PMCID: PMC275636
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31817

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