Homologous sequences in adenovirus E1A and human papillomavirus E7 proteins mediate interaction with the same set of cellular proteins

Dyson, N., Guida, P., Munger, K., Harlow, E. (December 1992) Homologous sequences in adenovirus E1A and human papillomavirus E7 proteins mediate interaction with the same set of cellular proteins. J Virol, 66 (12). pp. 6893-902. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1331501

Abstract

Studies of adenovirus E1A oncoprotein mutants suggest that the association of E1A with the retinoblastoma protein (pRB) is necessary for E1A-mediated transformation. Mutational analysis of E1A indicates that two regions of pRB are required for E1A to form stable complexes with the retinoblastoma protein. In addition to pRB binding, these regions are necessary for E1A association with several other cellular proteins, including p130, p107, cyclin A, and p33cdk2. Here we show that short synthetic peptides containing the pRB-binding sequences of E1A are sufficient for interaction with p107, cyclin A, and p130. The E7 protein of human papillomavirus type 16 contains an element that binds to pRB and appears to be functionally homologous to the E1A sequences. Peptides containing this region of the E7 protein were able to interact with p107, cyclin A, and p130 in addition to pRB. These findings suggest that the common mechanism of transformation used by these viral oncogenes involves their association with a set of polypeptides.

Item Type: Paper
Uncontrolled Keywords: Adenovirus E1A Proteins/genetics/isolation & purification/*metabolism Adenoviruses, Human/genetics/*metabolism Amino Acid Sequence Binding, Competitive HeLa Cells Humans Molecular Sequence Data Neoplasm Proteins/isolation & purification/*metabolism Oncogene Proteins, Viral/genetics/isolation & purification/*metabolism Papillomaviridae/genetics/*metabolism Papillomavirus E7 Proteins Peptide Mapping Peptides/chemical synthesis/pharmacology Sequence Homology, Amino Acid Tumor Cells, Cultured
Subjects: organism description > virus > adenovirus
diseases & disorders > viral diseases > human papillomavirus
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: December 1992
Date Deposited: 30 Sep 2015 19:36
Last Modified: 30 Sep 2015 19:36
PMCID: PMC240306
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31808

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