Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins

Xiong, Y., Menninger, J., Beach, D., Ward, D. C. (July 1992) Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. Genomics, 13 (3). pp. 575-84. ISSN 0888-7543 (Print)0888-7543 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1386336
DOI: 10.1016/0888-7543(92)90127-E

Abstract

A human D-type cyclin gene (CCND1/cyclin D1/PRAD1) was previously isolated by virtue of its ability to complement a triple G1 cyclin (Cln) deficiency of Saccharomyces cerevisiae and was also identified as a candidate BCL1 oncogene. We now report the molecular cloning of two additional human D-type cyclin genes, CCND2 (cyclin D2) and CCND3 (cyclin D3). All three human D-type cyclin genes encode small (33-34 kDa) proteins that share an average of 57% identity over the entire coding region and 78% in the cyclin box. The D-type cyclins are most closely related to cyclin A (39% identity) and cyclin E (36%), followed by cyclin B (29%) and cyclin C (21%). Isolation and characterization of genomic clones revealed two pseudogenes corresponding to CCND2 and CCND3, respectively. All three cyclin D genes are interrupted by an intron at the same position. CCND2 has been mapped to chromosome 12p13, and CCND3 has been mapped to chromosome 6p21.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Base Sequence Chromosome Mapping Cloning, Molecular Cyclins/*genetics DNA/genetics Humans Mice Molecular Sequence Data Pseudogenes Sequence Homology, Nucleic Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
Investigative techniques and equipment > cloning
Investigative techniques and equipment > assays > cloning
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: July 1992
Date Deposited: 30 Sep 2015 16:07
Last Modified: 30 Sep 2015 16:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31801

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