Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter

Tanaka, M., Lai, J. S., Herr, W. (1992) Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter. Cell, 68 (4). pp. 755-67. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1739980
DOI: 10.1016/0092-8674(92)90150-B

Abstract

The promoter specificity of transcriptional activators is generally thought to be conferred by the specificity of the DNA-binding domain, which brings the activation domain to the appropriate promoter sequence. We show here, however, that Oct-1 and Oct-2 can differentially activate transcription not through DNA binding specificity but instead through the use of promoter-selective activation domains. These distinct activation domains lead to stimulation of the U2 small nuclear RNA promoter by Oct-1 and an mRNA promoter by Oct-2. An Oct-2 variant, called Oct-2B, differs from Oct-2 by an Oct-1-related C-terminal extension that results from alternative splicing. This variant gains the ability to activate the U2 small nuclear RNA promoter. Thus, the promoter selectivity of a transcriptional activator can be changed, in this case by alternative splicing, without affecting its DNA binding specificity.

Item Type: Paper
Uncontrolled Keywords: DNA-Binding Proteins/genetics Gene Expression Regulation HeLa Cells Humans Models, Genetic *Promoter Regions, Genetic RNA Splicing RNA, Messenger/*genetics RNA, Small Nuclear/*genetics Trans-Activators/*genetics Transfection
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > snRNA
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date Deposited: 01 Oct 2015 20:05
Last Modified: 01 Oct 2015 20:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31781

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