Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity

Mizukami, Y., Ma, H. (October 1992) Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell, 71 (1). pp. 119-31. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1356630
DOI: 10.1016/0092-8674(92)90271-D

Abstract

The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP2), result in the replacement of the perianth organs by the reproductive organs. On the basis of these observations, it has been proposed that AG and AP2 act in an antagonistic fashion. To test this hypothesis, we have studied the effects of ectopically expressed AG in transgenic Arabidopsis plants. The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis/*genetics/growth & development/ultrastructure Base Sequence Cloning, Molecular Gene Expression/genetics Genes, Homeobox/*genetics *Genes, Plant Microscopy, Electron, Scanning Molecular Sequence Data Mutagenesis/genetics Plant Proteins/*genetics Plants, Genetically Modified/*genetics Plasmids/genetics Polymerase Chain Reaction Recombinant Fusion Proteins/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > plant
organism description > plant behavior > plant orientation > plant polarity
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic plants
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 2 October 1992
Date Deposited: 01 Oct 2015 20:01
Last Modified: 01 Oct 2015 20:01
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31780

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