A Tbp Complex Essential for Transcription from Tata-Less but Not Tata-Containing Rna Polymerase-Iii Promoters Is Part of the Tfiiib Fraction

Lobo, S. M., Tanaka, M., Sullivan, M. L., Hernandez, N. (December 1992) A Tbp Complex Essential for Transcription from Tata-Less but Not Tata-Containing Rna Polymerase-Iii Promoters Is Part of the Tfiiib Fraction. Cell, 71 (6). pp. 1029-1040. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/1458534
DOI: 10.1016/0092-8674(92)90397-U

Abstract

The TATA box-binding protein TBP directs transcription by all three eukaryotic RNA polymerases. In mammalian cells, TBP is found in at least three different complexes: SL1, D-TFIID, and B-TFIID. While SL1 and D-TFIID are involved in RNA polymerase I and II transcription, respectively, no unique function has been assigned to the B-TFIID complex. Here we show that the TFIIIB fraction required for RNA polymerase III transcription contains two separable components, one of which is a TBP-containing complex that may correspond to B-TFIID. For transcription of TATA-less RNA polymerase III genes such as the VAI, 5S, and 7SL genes, this complex cannot be replaced by either TBP alone or the D-TFIID complex. In contrast, TBP alone is active for basal transcription from the TATA-containing U6 promoter. This indicates different requirements for recruiting TBP to TATA-less and TATA-containing RNA polymerase III promoters.

Item Type: Paper
Uncontrolled Keywords: HUMAN U6 GENE SACCHAROMYCES-CEREVISIAE ACCURATE TRANSCRIPTION MULTIPLE FACTORS BINDING-PROTEIN SNRNA PROMOTER FACTOR-TAU SCHIZOSACCHAROMYCES-POMBE FUNCTIONAL DOMAINS FACTOR-EPSILON
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date: December 1992
Date Deposited: 01 Oct 2015 19:40
Last Modified: 01 Oct 2015 19:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31778

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