Analysis of embryonic mouse development: construction of a high-resolution, two-dimensional gel protein database

Latham, K. E., Garrels, J. I., Chang, C., Solter, D. (1992) Analysis of embryonic mouse development: construction of a high-resolution, two-dimensional gel protein database. Appl Theor Electrophor, 2 (6). pp. 163-70.

URL: http://www.ncbi.nlm.nih.gov/pubmed/1567917

Abstract

Numerous studies have revealed stage specific alterations in protein synthesis that occur in mouse embryos. A thorough analysis of these changes has been hampered by limitations in the ability to resolve individual proteins, the ability to accurately quantify and manage data from two-dimensional gel images, and by variation in the staging of the embryos used. To learn more of the changes in protein synthesis that occur during early development, we constructed a protein database for the mouse embryo using the QUEST system of high-resolution, two-dimensional gel electrophoresis and computerized gel image analysis (Garrels, 1989, J. Biol. Chem., 264:526). Synchronous cohorts of embryos were labeled at 3 h intervals throughout the entire preimplantation period from fertilization to blastocyst stage in order to characterize in detail the changes in protein synthesis pattern that occur during normal preimplantation development. Additional samples were prepared from early post-implantation embryos, isolated inner cell mass and trophoblast cells, and cultured embryonic stem cells. These provide the means for identifying cell and tissue specific proteins and for characterizing changes in protein synthesis that accompany early cellular differentiation in the embryo. We present here a description of the mouse embryo database and discuss its potential usefulness to the study of mammalian embryogenesis.

Item Type: Paper
Uncontrolled Keywords: Animals Culture Techniques *Databases, Factual Electrophoresis, Gel, Two-Dimensional Embryo, Mammalian/*physiology Embryonic and Fetal Development/*physiology Mice Mice, Inbred Strains/*embryology Molecular Weight Protein Biosynthesis Proteins/*analysis
Subjects: organism description > animal > developmental stage
organism description > animal > developmental stage > embryo
Investigative techniques and equipment > assays > gel electrophoresis
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1992
Date Deposited: 01 Oct 2015 18:26
Last Modified: 01 Oct 2015 18:26
URI: http://repository.cshl.edu/id/eprint/31771

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