The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response

Sheltzer, J. M., Rose, M. D. (2009) The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response. Mol Biol Cell, 20 (12). pp. 2909-19. ISSN 1939-4586 (Electronic)1059-1524 (Linking)

[img]
Preview
PDF (Paper)
Sheltzer Molecular Biol Cell 2009.pdf - Published Version

Download (1934Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/19403698
DOI: 10.1091/mbc.E08-09-0923

Abstract

Mating yeast cells remove their cell walls and fuse their plasma membranes in a spatially restricted cell contact region. Cell wall removal is dependent on Fus2p, an amphiphysin-associated Rho-GEF homolog. As mating cells polarize, Fus2p-GFP localizes to the tip of the mating projection, where cell fusion will occur, and to cytoplasmic puncta, which show rapid movement toward the tip. Movement requires polymerized actin, whereas tip localization is dependent on both actin and a membrane protein, Fus1p. Here, we show that Fus2p-GFP movement is specifically dependent on Myo2p, a type V myosin, and not on Myo4p, another type V myosin, or Myo3p and Myo5p, type I myosins. Fus2p-GFP tip localization and actin polarization in shmoos are also dependent on Myo2p. A temperature-sensitive tropomyosin mutation and Myo2p alleles that specifically disrupt vesicle binding caused rapid loss of actin patch organization, indicating that transport is required to maintain actin polarity. Mutant shmoos lost actin polarity more rapidly than mitotic cells, suggesting that the maintenance of cell polarity in shmoos is more sensitive to perturbation. The different velocities, differential sensitivity to mutation and lack of colocalization suggest that Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles.

Item Type: Paper
Uncontrolled Keywords: Actins/*metabolism Alleles *Cell Polarity Cytoskeletal Proteins/*metabolism Cytoskeleton/metabolism *Genes, Mating Type, Fungal Membrane Proteins/*metabolism Mutation/genetics Myosin Heavy Chains/chemistry/*metabolism Myosin Type V/chemistry/*metabolism Protein Structure, Tertiary Protein Transport Saccharomyces cerevisiae/*cytology/*metabolism Saccharomyces cerevisiae Proteins/chemistry/*metabolism Secretory Vesicles/metabolism Tropomyosin/metabolism rab GTP-Binding Proteins/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell polarity
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > GTP binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > myosin
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Sheltzer lab
Depositing User: Matt Covey
Date Deposited: 10 Sep 2015 19:23
Last Modified: 10 Sep 2015 19:23
PMCID: PMC2695798
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31750

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving