Viral and Transgenic Reporters and Genetic Analysis of Adult Neurogenesis

Enikolopov, G., Overstreet-Wadiche, L., Ge, S. (August 2015) Viral and Transgenic Reporters and Genetic Analysis of Adult Neurogenesis. Cold Spring Harb Perspect Biol, 7 (8). ISSN 1943-0264 (Electronic)

URL: http://www.ncbi.nlm.nih.gov/pubmed/26238354
DOI: 10.1101/cshperspect.a018804

Abstract

Stem and progenitor cells of the developing and adult brain can be effectively identified and manipulated using reporter genes, introduced into transgenic reporter mouse lines or recombinant viruses. Such reporters rely on an ever-increasing variety of fluorescent proteins and a continuously expanding list of regulatory elements and of mouse lines engineered for cell- or time-specific recombination. An important extension of stem-cell-based genetic strategies is an opportunity to explore the properties of newly generated neurons and their contribution to synaptic plasticity. Here, we review available strategies for marking and quantifying various classes of stem and progenitor cells in the adult brain, genetically tracing their progeny, and studying the properties of stem cells and new neurons. We compare various experimental approaches to labeling and investigating stem cells and their progeny and discuss caveats and limitations inherent to each approach.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurogenesis
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date: 3 August 2015
Date Deposited: 06 Aug 2015 19:57
Last Modified: 06 Aug 2015 19:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31687

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