Barcoding cells using cell-surface programmable DNA-binding domains

Mali, P., Aach, J., Lee, J. H., Levner, D., Nip, L., Church, G. M. (May 2013) Barcoding cells using cell-surface programmable DNA-binding domains. Nat Methods, 10 (5). pp. 403-6. ISSN 1548-7105 (Electronic)1548-7091 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/23503053
DOI: 10.1038/nmeth.2407

Abstract

We report an approach to barcode cells through cell-surface expression of programmable zinc-finger DNA-binding domains (surface zinc fingers, sZFs). We show that sZFs enable sequence-specific labeling of living cells by dsDNA, and we develop a sequential labeling approach to image more than three cell types in mixed populations using three fluorophores. We demonstrate the versatility of sZFs through applications in which they serve as surrogate reporters, function as selective cell capture reagents and facilitate targeted cellular delivery of viruses.

Item Type: Paper
Uncontrolled Keywords: Cell Membrane/metabolism DNA Barcoding, Taxonomic Zinc Fingers
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
CSHL Authors:
Communities: CSHL labs > Lee lab
Depositing User: Matt Covey
Date: May 2013
Date Deposited: 22 Jul 2015 15:42
Last Modified: 22 Jul 2015 15:42
PMCID: PMC3641172
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31635

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