High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique

Pinskiy, V., Jones, J., Tolpygo, A. S., Franciotti, N., Weber, K., Mitra, P. P. (July 2015) High-Throughput Method of Whole-Brain Sectioning, Using the Tape-Transfer Technique. PLoS One, 10 (7). e0102363. ISSN 1932-6203 (Electronic)1932-6203 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/26181725
DOI: 10.1371/journal.pone.0102363

Abstract

Cryostat sectioning is a popular but labor-intensive method for preparing histological brain sections. We have developed a modification of the commercially available CryoJane tape collection method that significantly improves the ease of collection and the final quality of the tissue sections. The key modification involves an array of UVLEDs to achieve uniform polymerization of the glass slide and robust adhesion between the section and slide. This report presents system components and detailed procedural steps, and provides examples of end results; that is, 20mum mouse brain sections that have been successfully processed for routine Nissl, myelin staining, DAB histochemistry, and fluorescence. The method is also suitable for larger brains, such as rat and monkey.

Item Type: Paper
Subjects: Investigative techniques and equipment
organs, tissues, organelles, cell types and functions > organs types and functions > brain
Investigative techniques and equipment > brain atlas
CSHL Authors:
Communities: CSHL labs > Mitra lab
Depositing User: Matt Covey
Date: 16 July 2015
Date Deposited: 20 Jul 2015 19:32
Last Modified: 24 Aug 2015 19:30
PMCID: PMC4504703
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31631

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