piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis

Goh, W. S., Falciatori, I., Tam, O. H., Burgess, R., Meikar, O., Kotaja, N., Hammell, M., Hannon, G. J. (May 2015) piRNA-directed cleavage of meiotic transcripts regulates spermatogenesis. Genes Dev, 29 (10). pp. 1032-44. ISSN 1549-5477 (Electronic)0890-9369 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/25995188
DOI: 10.1101/gad.260455.115

Abstract

MIWI catalytic activity is required for spermatogenesis, indicating that piRNA-guided cleavage is critical for germ cell development. To identify meiotic piRNA targets, we augmented the mouse piRNA repertoire by introducing a human meiotic piRNA cluster. This triggered a spermatogenesis defect by inappropriately targeting the piRNA machinery to mouse mRNAs essential for germ cell development. Analysis of such de novo targets revealed a signature for pachytene piRNA target recognition. This enabled identification of both transposable elements and meiotically expressed protein-coding genes as targets of native piRNAs. Cleavage of genic targets began at the pachytene stage and resulted in progressive repression through meiosis, driven at least in part via the ping-pong cycle. Our data support the idea that meiotic piRNA populations must be strongly selected to enable successful spermatogenesis, both driving the response away from essential genes and directing the pathway toward mRNA targets that are regulated by small RNAs in meiotic cells.

Item Type: Paper
Uncontrolled Keywords: Miwi RNA cleavage exogenous piRNAs pachytene piRNAs ping-pong cycle spermatogenesis defect
Subjects: organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:
Communities: CSHL Cancer Center Shared Resources > Mass Spectrometry Service
CSHL Cancer Center Shared Resources > Proteomics Service
CSHL labs > Hammell M. lab
CSHL labs > Hannon lab
Watson School > Publications
Depositing User: Matt Covey
Date: 15 May 2015
Date Deposited: 12 Jun 2015 16:40
Last Modified: 03 Jan 2019 16:15
PMCID: PMC4441051
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31576

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