Selective up-Regulation of an Nmda Receptor Subunit Messenger-Rna in Cultured Cerebellar Granule Cells by K+-Induced Depolarization and Nmda Treatment

Bessho, Y., Nawa, H., Nakanishi, S. (January 1994) Selective up-Regulation of an Nmda Receptor Subunit Messenger-Rna in Cultured Cerebellar Granule Cells by K+-Induced Depolarization and Nmda Treatment. Neuron, 12 (1). pp. 87-95. ISSN 0896-6273

URL: http://www.ncbi.nlm.nih.gov/pubmed/8292362
DOI: 10.1016/0896-6273(94)90154-6

Abstract

High KCl or NMDA treatment promotes the survival of cultured neonatal cerebellar granule cells, and these cells become sensitive to NMDA toxicity after prolonged K+ depolarization. Following both treatments, the NMDA receptor increases, as assessed by fura-2 fluorescence analysis of NMDA receptor-mediated intracellular Ca2+ increase. Northern analysis indicates that both treatments specifically up-regulate NMDAR2A subunit mRNA through an increase in resting intracellular Ca2+ concentration. Antisense oligonucleotide analysis further indicates that NMDAR2A mRNA up-regulation is responsible for NMDA receptor induction. Our results demonstrate that regulation of a specific NMDA receptor subunit mRNA governs NMDA receptor induction, which is thought to play an important role in granule cell survival and death.

Item Type: Paper
Uncontrolled Keywords: GLUTAMATE RECEPTORS MOLECULAR DIVERSITY EXPRESSION DISTINCTION SURVIVAL SUBTYPES NEURONS BRAIN
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > NMDA receptor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: January 1994
Date Deposited: 04 May 2015 15:57
Last Modified: 04 May 2015 15:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31480

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