Inhibition of G1 cyclin activity by the Ras/cAMP pathway in yeast

Tokiwa, G., Tyers, M., Volpe, T., Futcher, B. (September 1994) Inhibition of G1 cyclin activity by the Ras/cAMP pathway in yeast. Nature, 371 (6495). pp. 342-5. ISSN 0028-0836 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8090204
DOI: 10.1038/371342a0

Abstract

In the yeast Saccharomyces cerevisiae, commitment to cell division (Start) requires growth to a critical cell size. The G1 cyclins Cln1, Cln2 and Cln3 activate the Cdc28 protein kinase and are rate-limiting activators of Start. When glucose is added to cells growing in a poor carbon source, the critical cell size required for Start is reset from a small to a large size. In yeast, glucose acts through Ras proteins to stimulate adenylyl cyclase, activating the three cyclic AMP-dependent protein kinases Tpk1, Tpk2 and Tpk3 (refs 8, 9). We find that stimulation of the Ras/cAMP pathway represses expression of CLN1, CLN2 and co-regulated genes, inhibiting Start. This helps explain the increase in critical size when cells are shifted from poor to rich medium. This connection between the molecules controlling growth (Ras/cAMP) and those controlling division (cyclins) helps explain how division is co-ordinated with growth.

Item Type: Paper
Uncontrolled Keywords: Cell Division/genetics/physiology Cyclic AMP/ metabolism Cyclic AMP-Dependent Protein Kinases/metabolism Cyclins/ antagonists & inhibitors/metabolism Fungal Proteins/ metabolism G1 Phase/genetics/physiology Gene Expression Regulation, Fungal Glucose/metabolism Mutation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Saccharomyces cerevisiae/ cytology/genetics/metabolism Signal Transduction ras Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor > Cyclic AMP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Futcher lab
Depositing User: Matt Covey
Date: 22 September 1994
Date Deposited: 05 May 2015 20:51
Last Modified: 05 May 2015 20:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31454

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving