Brain-derived neurotrophic factor promotes differentiation of striatal GABAergic neurons

Mizuno, K., Carnahan, J., Nawa, H. (1994) Brain-derived neurotrophic factor promotes differentiation of striatal GABAergic neurons. Dev Biol, 165 (1). pp. 243-56. ISSN 0012-1606 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8088442
DOI: 10.1006/dbio.1994.1250

Abstract

The influence of neurotrophins on GABAergic properties of developing striatal neurons was investigated both in vivo and in vitro. Brain-derived neurotrophic factor (BDNF) specifically elevated cellular GABA content in striatal culture without altering neuronal survival. Neurotrophin-5 produced a similar effect on GABA, but nerve growth factor and neurotrophin-3 had no effect. An increase in GABA content in the striatum was also observed following BDNF injections into the cerebroventricle of neonatal rats. The increase of GABA levels in culture mainly resulted from an increase in holoenzyme activity of the GABA synthetic enzyme glutamic acid decarboxylase (GAD) and elevation of GABA uptake activity. In BDNF-treated striatal cultures, the newly differentiated neurons extended elaborate neurites and exhibited strong GAD immunoreactivity. These alterations were presumably caused by the upregulation of mRNA encoding GAD67 and the neuronal GABA transporter GAT-1. BDNF treatment also promoted other phenotypic differentiation of striatal neurons: BDNF increased the frequency of parvalbumin-immunoreactive neurons and calbindin-immunoreactive neurons and neuropeptide content for neuropeptide Y and somatostatin. These observations suggest that neurotrophins may contribute to phenotypic differentiation of GABAergic neurons in the developing striatum.

Item Type: Paper
Uncontrolled Keywords: Animals Animals, Newborn Brain-Derived Neurotrophic Factor Cell Differentiation/drug effects Cells, Cultured Cerebral Cortex/cytology/embryology/metabolism Corpus Striatum/cytology/embryology/ metabolism Glutamate Decarboxylase/metabolism Nerve Growth Factors/ physiology Nerve Tissue Proteins/ physiology Neurons/ cytology/metabolism Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't gamma-Aminobutyric Acid/biosynthesis/ metabolism
Subjects: bioinformatics > genomics and proteomics > small molecules > GABAergic
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > brain derived neurotrophic factor
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions > tissues types and functions > striatum
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 05 May 2015 19:52
Last Modified: 05 May 2015 19:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31453

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