Expression, purification, and crystallization of restriction endonuclease PvuII with DNA containing its recognition site

Balendiran, K., Bonventre, J., Knott, R., Jack, W., Benner, J., Schildkraut, I., Anderson, J. E. (1994) Expression, purification, and crystallization of restriction endonuclease PvuII with DNA containing its recognition site. Proteins, 19 (1). pp. 77-9. ISSN 0887-3585 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8066089
DOI: 10.1002/prot.340190110

Abstract

We have overexpressed the type II restriction endonuclease PvuII (R-PvuII) in E. coli, prepared large amounts of the homogeneous enzyme, and crystallized it with an oligonucleotide carrying a PvuII recognition site. The cocrystals are orthorhombic space group P2(1)2(1)2(1) with cell constants a = 95.8 A, b = 86.3 A, c = 48.5 A, and diffract X-rays to at least 2.7 A. There is a complex of two protein subunits and one oligonucleotide duplex in the asymmetric unit.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Binding Sites Crystallization Crystallography, X-Ray Deoxyribonucleases, Type II Site-Specific/ chemistry/isolation & purification/metabolism Escherichia coli Gene Expression Molecular Sequence Data Oligodeoxyribonucleotides/ metabolism Recombinant Fusion Proteins/isolation & purification/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
Investigative techniques and equipment > x ray crystallography
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 05 Aug 2015 19:45
Last Modified: 05 Aug 2015 19:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31450

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