Regulation of Neuropeptides in Adult-Rat Forebrain by the Neurotrophins Bdnf and Ngf

Croll, S. D., Wiegand, S. J., Anderson, K. D., Lindsay, R. M., Nawa, H. (August 1994) Regulation of Neuropeptides in Adult-Rat Forebrain by the Neurotrophins Bdnf and Ngf. European Journal of Neuroscience, 6 (8). pp. 1343-1353. ISSN 0953-816X

URL: http://www.ncbi.nlm.nih.gov/pubmed/7981876
DOI: 10.1111/j.1460-9568.1994.tb00325.x

Abstract

The expression of neuropeptides and neurotrophic factors is altered in the hippocampus after seizure induction in rats. Because the increase in brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) mRNAs precede changes in neuropeptide expression after seizure, it is possible that BDNF and NGF mediate subsequent alterations in peptide expression. To test this hypothesis directly, BDNF or NGF was infused into the hippocampus and cortex of adult rats. To ascertain the regional specificity of any observed effects of neurotrophin administration on neuropeptide expression, infusions into the striatum were also studied. To control for specificity, vehicle was also infused into the same sites. Peptide and mRNA alterations were assessed by Northern analysis, immunohistochemistry and radioimmunoassay. BDNF produced elevations of peptide and mRNA for neuropeptide Y and cholecystokinin in hippocampus and cortex, and somatostatin in cortex. BDNF increased mRNAs for neuropeptide Y, cholecystokinin, substance P and dynorphin in striatum. In contrast, BDNF decreased dynorphin peptide and mRNA in hippocampus. NGF's effects were limited to small mRNA increases, without corresponding changes in peptide levels, for neuropeptide Y in hippocampus and striatum, substance P in cortex and cholecystokinin in striatum. The distinct and limited effects of NGF infusion on neuropeptide expression demonstrate that BDNF's effects are not non-specific results of protein infusion into the brain. These findings indicate that BDNF may play a regionally specific role in modulating neuropeptide expression in the normal brain as well as in various pathophysiological states.

Item Type: Paper
Uncontrolled Keywords: NEUROTROPHIC FACTORS NEUROPEPTIDE Y DYNORPHIN SOMATOSTATIN CHOLECYSTOKININ NERVE GROWTH-FACTOR ENKEPHALIN-LIKE IMMUNOREACTIVITY MESSENGER-RNA EXPRESSION MOLECULAR-CLONING HIPPOCAMPAL-NEURONS CORTICAL-NEURONS LIMBIC SEIZURES RAPID INCREASE BRAIN RECEPTOR
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > brain derived neurotrophic factor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: August 1994
Date Deposited: 26 Aug 2015 15:07
Last Modified: 26 Aug 2015 15:07
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31432

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving