Localization of Differentially Phosphorylated Isoforms of Microtubule-Associated Protein 1b in Cultured Rat Hippocampal-Neurons

Ulloa, L., Diezguerra, F. J., Avila, J., Diaznido, J. (1994) Localization of Differentially Phosphorylated Isoforms of Microtubule-Associated Protein 1b in Cultured Rat Hippocampal-Neurons. Neuroscience, 61 (2). pp. 211-223. ISSN 0306-4522

URL: http://www.ncbi.nlm.nih.gov/pubmed/7969903
DOI: 10.1016/0306-4522(94)90225-9

Abstract

The development and plasticity of axons and dendrites in mammalian neurons may depend on the presence and phosphorylation state of cytoskeletal proteins, including certain microtubule-associated proteins. One of these proteins, microtubule-associated protein 1B, is modified by different protein kinases, which give rise to two major types of phosphorylated isoforms. The distribution of these isoforms in cultured hippocampal neurons has been studied using antibodies to specific phosphorylation-sensitive epitopes. Mode I-phosphorylated MAP1B is largely restricted to developing axonal processes, particularly at their distal regions including their growth cones where no mode I-dephosphorylated MAP1B is present. Axonal maturation is accompanied by dephosphorylation of MAP1B at mode I sites. Thus, mode I-phosphorylated MAP1B may be a marker for axonal growth. In contrast, mode II-phosphorylated MAP1B is abundant in the axonal and somatodendritic compartments, and no increased dephosphorylation occurs during maturation. These results are compatible with a role for the mode I phosphorylation of MAP1B (which might be catalysed by proline-directed protein kinases) in supporting a rapid axonal-specific growth mechanism and a more general role for the mode II phosphorylation of MAP1B (which seems to be catalysed by casein kinase II) in controlling axonal and dendritic growth and remodeling.

Item Type: Paper
Uncontrolled Keywords: CENTRAL-NERVOUS-SYSTEM CASEIN KINASE-II BRAIN-DEVELOPMENT ALZHEIMERS-DISEASE GROWTH CONES EXPRESSION POLARITY TAU MAP1B MAP-1B
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 25 Aug 2015 19:08
Last Modified: 25 Aug 2015 19:08
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31429

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