Early Changes in Protein-Synthesis Induced by Basic Fibroblast Growth-Factor, Nerve Growth-Factor, and Epidermal Growth-Factor in Pc12 Pheochromocytoma Cells

Hondermarck, H., McLaughlin, C. S., Patterson, S. D., Bradshaw, R. A. (September 1994) Early Changes in Protein-Synthesis Induced by Basic Fibroblast Growth-Factor, Nerve Growth-Factor, and Epidermal Growth-Factor in Pc12 Pheochromocytoma Cells. Proceedings of the National Academy of Sciences of the United States of America, 91 (20). pp. 9377-9381. ISSN 0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/7937773

Abstract

Nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) stimulate neuronal differentiation, whereas epidermal growth factor (EGP) promotes only mitogenic responses in PC12 pheochromocytoma cells. The early changes in protein synthesis induced by bFGF, NGF, and EGF in these cells have been determined by two-dimensional PAGE of [S-35]methionine-labeled proteins and computerized image analysis. The rate of synthesis of only 29 proteins (out of approximate to 1500 identified) was found to be modulated during the first several hours of growth factor stimulation. Individually, 12 were affected by EGF, 23 were affected by bFGF, and 20 were affected by NGF. Eight of these were regulated by all three growth factors, while 10 proteins were commonly induced by bFGF and NGF, in accordance with the essentially identical morphological responses induced by these two factors. In addition, the effects of bFGF and NGF were about equally divided between increases and decreases in the rate of synthesis of individual proteins, whereas EGF caused significantly more positive (increased) responses. All proteins modulated by NGF or FGF alone were negative in their response and those induced by only EGF were positive. Of particular interest, the rate of synthesis of two proteins of 55 kDa and pI 5.45 and 5.50 was dramatically and transiently induced during the first 2 hr of bFGF and NGF treatment and was not affected by EGF. This study indicates that all three factors elicit early increases and decreases in the synthesis of a quite limited number of proteins and provides molecular evidence for the specificity of a differentiative vs. a proliferative growth factor-induced signaling pathway in these cells.

Item Type: Paper
Uncontrolled Keywords: NEURITE OUTGROWTH NEURONAL DIFFERENTIATION TYROSINE PHOSPHORYLATION ORNITHINE DECARBOXYLASE GEL-ELECTROPHORESIS MAP KINASE ACTIVATION INDUCTION EXPRESSION PC12-CELLS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > epidermal growth factor
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > fibroblast growth factor
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: September 1994
Date Deposited: 06 Aug 2015 19:13
Last Modified: 06 Aug 2015 19:13
PMCID: PMC44815
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31418

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