Diaz-Nido, J., Mizuno, K., Nawa, H., Marshak, D. R.
(1994)
Regulation of protein kinase CK2 isoform expression during rat brain development.
Cell Mol Biol Res, 40 (5-6).
pp. 581-5.
ISSN 0968-8773 (Print)
Abstract
Protein kinase CK2 is very abundant in rat brain when compared with other rat tissues. The enzyme is an oligomeric protein with the structures alpha 2 beta 2, alpha alpha'beta 2 and alpha'2 beta 2. The alpha and alpha' subunits are catalytic and have a high degree of homology, whereas the beta subunit seems regulatory. Using specific antibodies to synthetic peptide antigens that discriminate among the alpha, alpha', and beta subunits of CK2, we have observed a significant increase in the amount of alpha' subunit during the late postnatal neocortical maturation period. The increased alpha' expression occurs at a parallel time to synaptogenesis. As for its distribution, the alpha' subunit of CK2 is much more abundant in neurons (particularly in large size neurons) than it is in glia. These results are consistent with a hypothetical role for CK2 isoforms containing alpha' subunits in the regulation of specific functions in fully differentiated neurons.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Amino Acid Sequence
Animals
Casein Kinase II
Cells, Cultured
Cerebral Cortex/embryology/ enzymology/growth & development
Enzyme Induction
Gene Expression Regulation, Developmental
Molecular Sequence Data
Nerve Tissue Proteins/ biosynthesis/genetics
Neuroglia/enzymology
Neurons/enzymology
Peptides/chemical synthesis/metabolism
Protein Conformation
Protein-Serine-Threonine Kinases/ biosynthesis/chemistry/genetics
Rats
Research Support, Non-U.S. Gov't |
| Subjects: |
organs, tissues, organelles, cell types and functions > organs types and functions > brain
organism description > animal > developmental stage
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat |
| CSHL Authors: |
|
| Communities: |
CSHL labs |
| Depositing User: |
Matt Covey
|
| Date: |
1994 |
| Date Deposited: |
28 Aug 2015 14:51 |
| Last Modified: |
28 Aug 2015 14:51 |
| URI: |
https://repository.cshl.edu/id/eprint/31402 |
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