Chicken Antibodies to Rabbit Muscle Actin with a Restricted Repertoire of F-Actin Recognition

Schrader, M., Temmgrove, C. J., Lessard, J. L., Jockusch, B. M. (April 1994) Chicken Antibodies to Rabbit Muscle Actin with a Restricted Repertoire of F-Actin Recognition. European Journal of Cell Biology, 63 (2). pp. 326-335. ISSN 0171-9335

URL: http://www.ncbi.nlm.nih.gov/pubmed/7521836

Abstract

This report describes a polyclonal antibody against actin with unexpected and unusual properties. The antibody was raised in chicken immunized with a complex of DNase I and rabbit skeletal muscle actin, and purified from egg yolk by affinity chromatography. In Western blots, it reacted with alpha, beta and gamma isoforms of actin. In immunofluorescence and dot blot assays, however, it recognized selectively actin filaments in myofibrils, microvilli of brush border-type epithelium and the ''comet tails'' of the intracellular parasite Listeria monocytogenes, while it did not react with stress fibers and peripheral belts of fibroblasts and epithelial cells, respectively. This reactivity pattern is reminiscent of that previously described for a monoclonal mouse antibody raised against smooth muscle actin (Sawtell et al., Cell Moth. Cytoskel. 11, 318, 1988). The data presented in this study are consistent with the hypothesis that the chicken antibody recognizes an actin epitope/actin epitopes either accessible in only a subpopulation of microfilaments, or expressed only in a particular conformation of F-actin.

Item Type: Paper
Uncontrolled Keywords: ACTIN CONFORMATION ANTIACTIN BRUSH BORDER ACTIN CHICKEN ANTIBODIES EGG-YOLK POLYCLONAL ANTIBODIES IMMUNIZED HENS PROTEINS LISTERIA COMPLEX
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > antibodies
CSHL Authors:
Communities: CSHL labs > Helfman lab
Depositing User: Matt Covey
Date: April 1994
Date Deposited: 27 Aug 2015 21:03
Last Modified: 27 Aug 2015 21:03
URI: http://repository.cshl.edu/id/eprint/31398

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