Intraventricular Administration of Bdnf Increases Neuropeptide Expression in Newborn Rat-Brain

Nawa, H., Pelleymounter, M. A., Carnahan, J. (1994) Intraventricular Administration of Bdnf Increases Neuropeptide Expression in Newborn Rat-Brain. Journal of Neuroscience, 14 (6). pp. 3751-3765. ISSN 0270-6474

URL: http://www.ncbi.nlm.nih.gov/pubmed/7515953

Abstract

Brain-derived neurotrophic factor (BDNF) specifically enhances and maintains the expression of neuropeptide Y (NPY) and somatostatin (SOM) in cultured neocortical neurons (Nawa et al., 1993). In this article, we examined its effects in vivo on neuropeptide expression in various brain regions by injecting BDNF into the cerebroventricle of newborn rats. Repeated administration (2 x) of BDNF increased contents of NPY-like immunoreactivity (NPY-LI) and substance P (SP)-LI most markedly in the anterior neocortex by 11- and 24-fold, respectively, in comparison to values in the animals receiving control injection. A smaller but significant increase was also observed in immunoreactivity for somatostatin (SOM), enkephalin (ENK), and cholecystokinin (CCK). mRNA for NPY, SP, and SOM was similarly upregulated in the anterior neocortex, suggesting that BDNF enhances peptide synthesis rather than inhibiting peptide release or degradation. Among the brain regions examined, however, peptidergic responses to BDNF were different with respect to their spatial distribution and time course. Induction of SP-LI, NPY-LI, and SOM-LI around the injection site was most pronounced in cortical layers II/III, layers IV-VI, and layer VI, respectively. Peptidergic immunoreactivity was also enhanced in other brain regions ipsilateral to the injection site, for example, NPY-LI in the hippocampus, thalamic nuclei, and striatum, and SOM-LI in the striatum. A single injection of BDNF elevated SP-LI to a plateau level within 12 hr while NPY-LI and SOM-LI reached maximum levels at 48 hr, and then air returned to control levels at 68 hr. In contrast, the same dose of NGF had no influences on the neuropeptide levels at 48 hr. These observations suggest that BDNF regulates the development of neuropeptide expression in the CNS in a plastic manner.

Item Type: Paper
Uncontrolled Keywords: BRAIN-DERIVED NEUROTROPHIC FACTOR CEREBRAL CORTEX CHOLECYSTOKININ ENKEPHALIN NEUROPEPTIDE Y SUBSTANCE P SOMATOSTATIN NERVE GROWTH-FACTOR CILIARY NEUROTROPHIC FACTOR SEPTAL CHOLINERGIC NEURONS MESSENGER-RNA EXPRESSION TYROSINE PROTEIN-KINASE VASOACTIVE-INTESTINAL-PEPTIDE CULTURED SYMPATHETIC NEURONS ACID-INDUCED SEIZURES SUBSTANCE-P IMMUNOHISTOCHEMICAL ANALYSIS
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > brain derived neurotrophic factor
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 27 Aug 2015 20:45
Last Modified: 27 Aug 2015 20:45
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31396

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