Mapping Social Behavior-Induced Brain Activation at Cellular Resolution in the Mouse

Kim, Yongsoo, Venkataraju, Kannan Umadevi, Pradhan, Kith, Mende, Carolin, Taranda, Julian, Turaga, Srinivas C, Arganda-Carreras, Ignacio, Ng, Lydia, Hawrylycz, Michael J, Rockland, Kathleen S, Seung, H.  Sebastian, Osten, Pavel (2015) Mapping Social Behavior-Induced Brain Activation at Cellular Resolution in the Mouse. Cell Reports, 10 (2). pp. 292-305. ISSN 2211-1247

URL: http://www.ncbi.nlm.nih.gov/pubmed/25558063
DOI: 10.1016/j.celrep.2014.12.014

Abstract

Summary Understanding how brain activation mediates behaviors is a central goal of systems neuroscience. Here, we apply an automated method for mapping brain activation in the mouse in order to probe how sex-specific social behaviors are represented in the male brain. Our method uses the immediate-early-gene c-fos, a marker of neuronal activation, visualized by serial two-photon tomography: the c-fos-GFP+ neurons are computationally detected, their distribution is registered to a reference brain and a brain atlas, and their numbers are analyzed by statistical tests. Our results reveal distinct and shared female and male interaction-evoked patterns of male brain activation representing sex discrimination and social recognition. We also identify brain regions whose degree of activity correlates to specific features of social behaviors and estimate the total numbers and the densities of activated neurons per brain areas. Our study opens the door to automated screening of behavior-evoked brain activation in the mouse.

Item Type: Paper
Subjects: organism description > animal > mammal > rodent > mouse
Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:
Communities: CSHL labs > Osten lab
Stanley Institute for Cognitive Genomics
Depositing User: Matt Covey
Date Deposited: 30 Jan 2015 22:09
Last Modified: 06 Nov 2015 20:58
PMCID: PMC4294964
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31156

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