Gene regulation by CcpA and catabolite repression explored by RNA-Seq in Streptococcus mutans

Zeng, L., Choi, S. C., Danko, C. G., Siepel, A., Stanhope, M. J., Burne, R. A. (2013) Gene regulation by CcpA and catabolite repression explored by RNA-Seq in Streptococcus mutans. PLoS One, 8 (3). e60465. ISSN 1932-6203

[img]
Preview
PDF (Paper)
Siepel PLoS One 2013.pdf - Published Version

Download (1503Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/23555977
DOI: 10.1371/journal.pone.0060465

Abstract

A bacterial transcriptome of the primary etiological agent of human dental caries, Streptococcus mutans, is described here using deep RNA sequencing. Differential expression profiles of the transcriptome in the context of carbohydrate source, and of the presence or absence of the catabolite control protein CcpA, revealed good agreement with previously-published DNA microarrays. In addition, RNA-seq considerably expanded the repertoire of DNA sequences that showed statistically-significant changes in expression as a function of the presence of CcpA and growth carbohydrate. Novel mRNAs and small RNAs were identified, some of which were differentially expressed in conditions tested in this study, suggesting that the function of the S. mutans CcpA protein and the influence of carbohydrate sources has a more substantial impact on gene regulation than previously appreciated. Likewise, the data reveal that the mechanisms underlying prioritization of carbohydrate utilization are more diverse than what is currently understood. Collectively, this study demonstrates the validity of RNA-seq as a potentially more-powerful alternative to DNA microarrays in studying gene regulation in S. mutans because of the capacity of this approach to yield a more precise landscape of transcriptomic changes in response to specific mutations and growth conditions.

Item Type: Paper
Uncontrolled Keywords: Bacterial Proteins/*genetics *Catabolite Repression Dental Caries/*microbiology Galactose/metabolism *Gene Expression Regulation, Bacterial Glucose/metabolism Humans Mutation RNA, Bacterial/genetics RNA, Messenger/genetics Repressor Proteins/*genetics Streptococcus mutans/*genetics/growth & development/*metabolism Transcriptome
Subjects: organism description > bacteria
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

Investigative techniques and equipment > assays > RNA-seq
CSHL Authors:
Communities: CSHL labs > Siepel lab
Depositing User: Matt Covey
Date Deposited: 12 Jan 2015 16:49
Last Modified: 12 Jan 2015 16:49
PMCID: PMC3610829
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31116

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving