Brain stem cell division and maintenance studied using multi-isotope imaging mass spectrometry (MIMS)

Enikolopov, G., Guillermier, C., Wang, M., Trakimas, L., Steinhauser, M. L., Lechene, C. (November 2014) Brain stem cell division and maintenance studied using multi-isotope imaging mass spectrometry (MIMS). Surface and Interface Analysis, 46 (S1). pp. 140-143. ISSN 01422421

URL: http://onlinelibrary.wiley.com/doi/10.1002/sia.567...
DOI: 10.1002/sia.5675

Abstract

New neurons are continuously produced from neural stem cells in specific regions of the adult brain of animals and humans. In the hippocampus, a region crucial for cognitive function, neurogenesis responds to a multitude of extrinsic stimuli; emerging evidence indicates that it may be important for behavior, pathophysiology, brain repair, and response to drugs. We have developed an approach to identify and quantify the cellular targets of pro- and anti-neurogenic stimuli, based on reporter transgenicmouse lines in which neural stemand progenitor cells or their progeny aremarked by fluorescent proteins. Here, we demonstrate the feasibility of using multi-isotope imaging mass spectrometry for studying adult neurogenesis.

Item Type: Paper
Additional Information: Meeting Abstract
Uncontrolled Keywords: 15N-thymidine 81Br BrdU DNA synthesis Hippocampus MIMS Multi-isotope imagingmass spectrometry NanoSIMS Neurogenesis SIMS Stable isotope Stemcell division
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
Investigative techniques and equipment > spectroscopy > mass spectrometry
Publication Type > Meeting Abstract
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date: 1 November 2014
Date Deposited: 19 Dec 2014 18:02
Last Modified: 06 Feb 2018 16:14
URI: http://repository.cshl.edu/id/eprint/30979

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